Update of “Analysis of 15N incorporation into D-alanine: A new method for tracing nitrogen uptake by bacteria” (Veuger et al. 2005, Limnol. Oceanogr.: Methods 3: 230-240)

Bart Veuger, Jack J. Middelburg, Henricus T.S. Boschker,Marco Houtekamer

Limnol. Oceanogr. Methods 5:192-194 (2007) | DOI: 10.4319/lom.2007.5.192

ABSTRACT: After publication of the original manuscript, the presented method has been applied in various 15N- and 13C-labeling studies to trace incorporation of 15N- and/or 13C into bacterial and total microbial biomass in different coastal sediments (Veuger et al. 2006, Veuger 2006). These applications and analysis of the D-Ala content of some algal- and cyanobacterial cultures yielded valuable information regarding the interpretation of 15N and 13C incorporation into D-Ala and other hydrolysable amino acids (HAAs), which is presented in this update.