Detection of lysogeny in marine environments
Full Citation: Paul, J. H., and M. Weinbauer. 2010. Detection of lysogeny in marine environments, p. 30-33. In S. W. Wilhelm, M. G. Weinbauer, and C. A. Suttle [eds.], Manual of Aquatic Viral Ecology. ASLO. [DOI 10.4319/mave.2010.978-0-9845591-0-7.30]
ABSTRACT: Silent viral infections occur in all forms of life, from bacteria to humans, as indicated from genomic sequencing. Temperate phages can infect bacteria and establish a symbiotic relationship termed lysogeny, enabling the phage genome to be propagated in host daughter cells. The expression of prophage genes often results in an altered bacterial phenotype, often turning benign bacteria into virulent pathogens. The most widely used method to detect lysogens is to chemically induce their prophages and detect these via microscopy or flow cytometry. Although chemical induction is the gold standard in prophage detection, not all prophages can be detected by it. This review gives two methods for prophage induction in heterotrophic bacterioplankton, a method for induction of Synechococcus populations, and a method for isolating temperate phage, as well as a simple method to recognize prophage-like elements in bacterial genomes.